CANCER FACTS National Cancer Institute National Institutes of Health (NIH)

Key Points

  • A Pap test and pelvic exam are important parts of a woman’s routine health care because they can detect cancer or abnormalities that may lead to cancer of the cervix (see Question 3).
  • Women should have a Pap test at least once every 3 years, beginning about 3 years after they begin to have sexual intercourse, but no later than age 21 (see Question 6).
  • If the Pap test shows abnormalities, further tests and/or treatment may be necessary (see Question 11).
  • Human papillomavirus (HPV) infection is the primary risk factor for cervical cancer (see Question 13).

1. What is a pelvic exam?
In a pelvic exam, the uterus, vagina, ovaries, fallopian tubes, bladder, and rectum are felt to find any abnormality in their shape or size. During a pelvic exam, an instrument called a speculum is used to widen the vagina so that the upper portion of the vagina and the cervix can be seen.

2. What is a Pap test?
The Pap test (sometimes called a Pap smear) is a way to examine cells collected from the cervix (the lower, narrow end of the uterus). The main purpose of the Pap test is to find abnormal cell changes that may arise from cervical cancer or before cancer develops

3. Why are a Pap test and pelvic exam important?
A Pap test and pelvic exam are important parts of a woman’s routine health care because they can detect abnormalities that may lead to invasive cancer of the cervix. These abnormalities can be treated before cancer develops. Most invasive cancers of the cervix can be prevented if women have Pap tests regularly. Also, as with many types of cancer, cancer of the cervix is more likely to be treated successfully if it is detected early.

However, the American Cancer Society, the American College of Obstetricians and Gynecologists and the US Preventive Services Task Force all agree that for women who have a hysterectomy for benign reasons (that is, they did not have cancer), and they no longer have a cervix (the cervix was removed with the uterus), Paps smears may be discontinued. ACOG (the American College of Obstetricians and Gynecologist) adds that a woman should have had no prior history of moderate or severe dysplasia. Speak to your health care provider about whether this is an appropriate option for you.

These three groups also state that women over 70, with an intact cervix, may discontinue having Paps if they have had 3 consecutive normal Paps and they have had no abnormal pap tests in the last 10 years.

4. Who performs a Pap test?
Doctors and other specially trained health care professionals, such as physician assistants, nurse midwives, and nurse practitioners, may perform Pap tests and pelvic exams. These individuals are often called clinicians.

5. How is a Pap test done?
A Pap test is simple, quick, and painless; it can be done in a doctor’s office, a clinic, or a hospital. While a woman lies on an exam table, the clinician inserts a speculum into her vagina to widen it. A sample of cells is taken from the cervix with a wooden scraper and/or a small cervical brush. The specimen (or smear) is placed on a glass slide and preserved with a fixative, or is rinsed in a vial of fixative, and is sent to a laboratory for examination.

6. How often should a woman have a Pap test?
Women should talk with their clinician about when and how often they should have a Pap test. Current general guidelines recommend that women have a Pap test at least once every 3 years, beginning about 3 years after they begin to have sexual intercourse, but no later than age 21. Experts recommend waiting about 3 years after the start of sexual activity to avoid overtreatment for common, temporary abnormal changes. It is safe to wait 3 years, because cervical cancer usually develops slowly. Cervical cancer is extremely rare in women under age 25.

Women ages 65 to 70 who have had at least three normal Pap tests and no abnormal Pap tests in the last 10 years may decide, after talking with their clinician, to stop having Pap tests. Women who have had a hysterectomy (surgery to remove the uterus and cervix) do not need to have a Pap test, unless the surgery was done as a treatment for precancer or cancer.

7. When should the Pap test be done?
A woman should have this test when she is not menstruating; the best time is between 10 and 20 days after the first day of the last menstrual period. For about 2 days before a Pap test, she should avoid douching or using vaginal medicines or spermicidal foams, creams, or jellies (except as directed by a physician). These may wash away or hide abnormal cells.

8. How are the results of a Pap test reported?
Most laboratories in the United States use a standard set of terms called the Bethesda System to report test results. Under the Bethesda System, Pap test samples that have no cell abnormalities are reported as “negative for intraepithelial lesion or malignancy.” Samples with cell abnormalities are divided into the following categories:

ASC—atypical squamous cells. Squamous cells are the thin flat cells that form the surface of the cervix. The Bethesda System divides this category into two groups:

ASC–US—atypical squamous cells of undetermined significance. The squamous cells do not appear completely normal, but doctors are uncertain about what the cell changes mean. Sometimes the changes are related to HPV infection (see Question 13). ACS–US are considered mild abnormalities.

ASC–H—atypical squamous cells cannot exclude a high-grade squamous intraepithelial lesion. The cells do not appear normal, but doctors are uncertain about what the cell changes mean. ASC–H may be at higher risk of being precancerous.

AGC—atypical glandular cells. Glandular cells are mucus-producing cells found in the endocervical canal (opening in the center of the cervix) or in the lining of the uterus. The glandular cells do not appear normal, but doctors are uncertain about what the cell changes mean.

AIS—endocervical adenocarcinoma in situ. Precancerous cells are found in the glandular tissue.

LSIL—low-grade squamous intraepithelial lesion. Low-grade means there are early changes in the size and shape of cells. The word lesion refers to an area of abnormal tissue. Intraepithelial refers to the layer of cells that forms the surface of the cervix. LSILs are considered mild abnormalities caused by HPV infection.

HSIL—high-grade squamous intraepithelial lesion. High-grade means that there are more marked changes in the size and shape of the abnormal (precancerous) cells, meaning that the cells look very different from normal cells. HSILs are more severe abnormalities and have a higher likelihood of progressing to invasive cancer.

9. How common are Pap test abnormalities?
About 55 million Pap tests are performed each year in the United States. Of these, approximately 3.5 million (6 percent) are abnormal and require medical followup.

10. What do abnormal results mean?
A physician may simply describe Pap test results to a patient as “abnormal.” Cells on the surface of the cervix sometimes appear abnormal but are very rarely cancerous. It is important to remember that abnormal conditions do not always become cancerous, and some conditions are more likely to lead to cancer than others. A woman may want to ask her doctor for specific information about her Pap test result and what the result means.

There are several terms that may be used to describe abnormal results.

Dysplasia is a term used to describe abnormal cells. Dysplasia is not cancer, although it may develop into very early cancer of the cervix. The cells look abnormal under the microscope, but they do not invade nearby healthy tissue. There are four degrees of dysplasia, classified as mild, moderate, severe, or carcinoma in situ, depending on how abnormal the cells appear under the microscope. Carcinoma in situ means that cancer is present only in the layer of cells on the surface of the cervix, and has not spread to nearby tissues.

Squamous intraepithelial lesion (SIL) is another term that is used to describe abnormal changes in the cells on the surface of the cervix. The word squamous describes thin, flat cells that form the outer surface of the cervix. The word lesion refers to abnormal tissue. An intraepithelial lesion means that the abnormal cells are present only in the layer of cells on the surface of the cervix. A doctor may describe SIL as being low-grade (early changes in the size, shape, and number of cells) or high-grade (precancerous cells that look very different from normal cells).

Cervical intraepithelial neoplasia (CIN) is another term that is sometimes used to describe abnormal tissue findings. Neoplasia means an abnormal growth of cells. Intraepithelial refers to the layer of cells that form the surface of the cervix. The term CIN, along with a number (1 to 3), describes how much of the thickness of the lining of the cervix contains abnormal cells.

Atypical squamous cells are findings that are unclear, and not a definite abnormality.
Cervical cancer, or invasive cervical cancer, occurs when abnormal cells spread deeper into the cervix or to other tissues or organs.

11. What if Pap test results are abnormal?
If the Pap test shows an ambiguous or minor abnormality, the physician may repeat the test to determine whether further followup is needed. Many times, cell changes in the cervix go away without treatment. In some cases, doctors may prescribe estrogen cream for women who have ASC–US and are near or past menopause. Because these cell changes are often caused by low hormone levels, applying an estrogen cream to the cervix for a few weeks can usually help to clarify the cause of the cell changes.

If the Pap test shows a finding of ASC–H, LSIL, or HSIL, the physician may perform a colposcopy using an instrument much like a microscope (called a colposcope) to examine the vagina and the cervix. The colposcope does not enter the body. During a colposcopy, the physician may coat the cervix with a dilute vinegar solution that causes abnormal areas to turn white. The physician may also perform a biopsy (a biopsy is the removal of a small piece of tissue for study in a lab).

The physician may also perform endocervical curettage. This test involves scraping cells from inside the endocervical canal with a small spoon-shaped tool called a curette. The doctor may also remove a small piece of cervical tissue for examination. This procedure is called a biopsy. The cells or tissue are sent to a lab for study under a microscope.

If the lab finds abnormal cells that have a high chance of becoming cancer, further treatment is needed. Without treatment, these cells may turn into invasive cancer. Treatment options include the following:

LEEP (loop electrosurgical excision procedure) is surgery that uses an electrical current which is passed through a thin wire loop to act as a knife.
Cryotherapy destroys abnormal tissue by freezing it.
Laser therapy is the use of a narrow beam of intense light to destroy or remove abnormal cells.
Conization removes a cone-shaped piece of tissue using a knife, a laser, or the LEEP technique.

12. How do terms for Pap test abnormalities compare, and which tests and treatment options may be appropriate?
Pap Test Result Abbreviation Also Known As Tests and Treatments May Include
Atypical squamous cells–undetermined signficance ASC–US HPV testing Repeat Pap test Colposcopy and biopsy Estrogen cream
Atypical squamous cells–cannot exclude HSIL ASC–H Colposcopy and biopsy
Atypical glandular cells AGC Colposcopy and biopsy and/or endocervical curettage
Endocervical adenocarcinoma in situ AIS Colposcopy and biopsy and/or endocervical curettage
Low-grade squamous intraepithelial lesion LSIL Mild dysplasia or Cervical intraepithelial neoplasia–1 (CIN–1) Colposcopy and biopsy
High-grade squamous intraepithelial lesion HSIL Moderate dysplasia, Severe dysplasia, CIN–2, CIN–3, or Carcinoma in situ (CIS) Colposcopy and biopsy and/or endocervical curettage Further treatment with LEEP, cryotherapy, laser therapy, conization, or hysterectomy

13. How are human papillomaviruses (HPVs) associated with the development of cervical cancer?
Human papillomaviruses (HPVs) are a group of more than 100 viruses. Some types of HPV cause the common warts that grow on hands and feet. Some HPVs are sexually transmitted and cause wart-like growths on the genitals, but these types do not lead to cancer. More than a dozen other sexually transmitted HPVs have been linked to cervical cancer.

HPV infection is the primary risk factor for cervical cancer. However, although HPV infection is very common, only a very small percentage of women with untreated HPV infections develop cervical cancer.

14. Who is at risk for HPV infection?
HPV infection is more common in younger age groups, particularly among women in their late teens and twenties. Because HPVs are spread mainly through sexual contact, risk increases with number of sexual partners. Women who become sexually active at a young age, who have multiple sexual partners, and whose sexual partners have other partners are at increased risk. Women who are infected with the human immunodeficiency virus (HIV) are also at higher risk for being infected with HPVs and for developing cervical abnormalities. Nonsexual transmission of HPVs is rare. The virus often disappears but sometimes remains detectable for years after infection.

15. Does infection with a cancer-associated type of HPV always lead to a precancerous condition or cancer?
No. Most HPV infections appear to go away on their own without causing any kind of abnormality. However, persistent infection with cancer-associated HPV types increases the risk that mild abnormalities will progress to more severe abnormalities or cervical cancer. With regular followup care by trained clinicians, women with precancerous cervical abnormalities can be treated before cancer develops.

16. Have any studies been done to examine HPV testing and treatment options for mild Pap test abnormalities?
Findings of the ASCUS/LSIL Triage Study (ALTS), a major clinical trial (research study with people) funded and organized by the National Cancer Institute (NCI), suggest that HPV testing in women with ASC–US may help identify underlying abnormalities that need a doctor’s attention. The study results suggest that testing cervical samples for HPVs can identify which ASC–US abnormalities need treatment. A negative HPV test can provide reassurance that cancer or a precancerous condition is not present.

17. What are false positive and false negative results?
The Pap test is a screening test and, like any such test, it is not 100-percent accurate. Although false positive and false negative results do not occur very often, they can cause anxiety and can affect a woman’s health.

A false positive Pap test means that a patient is told she has abnormal cells, but the cells are actually normal. A false negative Pap test occurs when a specimen is called normal, but the woman has a significant abnormality that was missed. A false negative Pap test may delay the diagnosis and treatment of a precancerous condition. However, regular screening helps to compensate for the false negative result. If abnormal cells are missed at one time, chances are good that the cells will be detected the next time.

18. What methods are being developed to improve the accuracy of Pap tests?
In April 1996, the Consensus Development Conference on Cervical Cancer, which was convened by the National Institutes of Health (NIH), concluded that about half of false negative Pap tests are due to inadequate specimen collection. The other half are due to a failure to identify or interpret the specimens correctly. Although the conventional Pap test is effective in the majority of cases, the conference made it clear that new methods of collecting and reading specimens are needed to reduce the number of false negatives.

The Bethesda System requires laboratories to determine whether there are enough cervical cells in the specimen to make a proper evaluation. This requirement helps improve the quality of samples and sample collection. The Bethesda System requires a sample to be categorized as “satisfactory for evaluation” or “unsatisfactory for evaluation.”

One new method of collecting and analyzing samples is called liquid-based thin-layer slide preparation. This method may make it easier to screen for abnormal cells. Cervical cells are collected with a brush or other collection instrument. The instrument is rinsed in a vial of liquid preservative. The vial is sent to a laboratory, where an automated thin-layer slide device prepares the slide for viewing. Results of this method suggest that it is comparable to, or more sensitive than, standard Pap tests for the detection of significant abnormalities.

Computer image directed automated readers are also being used to improve the reading of Pap tests. This technology uses a microscope that conveys a cellular image to a computer, which analyzes the image for the presence of abnormal cells

19. Is there a vaccine against HPV or cervical cancer?
There are currently two HPV vaccines available.

    • Gardasil®. is vaccine available for both men and women. Developed by Merck, Gardasil® is close to 100% effective at preventing infection associated with HPV types 6 & 11(types associated with 90% of all genital warts) and types 16 &18 (types associated with 70% of all cervical cancers, and many vulvar and vaginal cancers).
    • Cervarix®, developed by GlaxoSmithKline (GSK) is a vaccine just for women. This vaccine is also close to 100% effective at preventing infection associated with HPV 16 & 18 (associated with 70% of all cervical cancers). Studies suggest Cervarix® also offers cross-protection against other “high risk” HPV types.

In 2006 the Food and Drug Administration (FDA) licensed Gardasil® for use with females ages 9-26 for the prevention of cervical pre-cancers and cancers, vulvar and vaginal pre-cancers, and genital warts. In 2009 the FDA approved Gardasil® for the prevention of genital warts in males ages 9-26.

The Advisory Committee on Immunization Practices (ACIP) – organized by the Centers for Disease Control and Prevention (CDC) to provide advice and guidance on the most effective means to prevent vaccine-preventable diseases – recommends routine use of Gardasil® for girls ages 11-12, with “catch up” immunization for women ages 13-26 who have not received the vaccine. Healthcare providers may give the vaccine to girls as young as age 9. ACIP recommends vaccination with Cervarix® for 11 and 12 year old girls, with “catch up” for those ages 13-25 who have not previously received the vaccine.
ACIP recommends “permissive use” (at the healthcare provider’s discretion) of Gardasil® with males 9-26.

Tested in thousands of people in many countries, both vaccines appear to be safe and well tolerated; the most common side effect has been soreness at the injection site.

20. I have been told I have Stage “O” adenocarcinoma cervical cancer. I already had a Cone biopsy. I want to still have children. My doctor wants to follow me up with regular Pap smears, is that OK?
Stage 0 adenocarcinoma is also known as adenocarcinoma “in situ”. If cone biopsies completely remove the tumor, then Pap follow up is acceptable and completely OK in women 30-35 or younger. If childbearing is strongly desired then follow-up with paps is OK if the risk of a recurrence is understood (the risk is directly related to the pathology speicmen and how far the tumor is from the edge of the resection margin. The hysterectomy should be eventually performed (after the disire for childbearing is concluded) as the long term risk of recurrence and progression of the cancer in the remaining cervix is unknown.

The next step is a review of the pathology specimens by an expert and an assessment of risk. Following that, there should be a conference between the pathologist and the gynecologist / gynecologic oncologist and then a plan formulated with the patient.


Leave a comment

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out / Change )

Twitter picture

You are commenting using your Twitter account. Log Out / Change )

Facebook photo

You are commenting using your Facebook account. Log Out / Change )

Google+ photo

You are commenting using your Google+ account. Log Out / Change )

Connecting to %s

Blog at WordPress.com.

%d bloggers like this: